Analysis of single nucleotide polymorphisms in major and candidate genes for production traits in Nero Siciliano pig breed
The breed is well adapted to marginal conditions and is appreciated for its reproductive performance, disease resistance
and production of tasty meat. For a genetic characterization of this breed we analyzed the allele frequencies of single
nucleotide polymorphisms (SNPs) in eight major or candidate genes (ryanodine receptor 1, RYR1; Na+, K+ ATPase subunit
α 2, ATP1A2; myosin heavy chain 2B, MYH4; sarcolipin, SLN; cathepsin B, CTSB; cystatin B, CSTB; estrogen receptor,
ESR; melanocortin receptor 1, MC1R) for performance and phenotypic traits. The animals that were sampled and
analyzed represent about 6-8% of the total NS pig population. PCR-RFLP or PCR-SSCP techniques were used to type the
DNA markers in the selected loci. Exact test of Hardy-Weinberg equilibrium was computed for each locus, Fis statistics
and heterozygosity were calculated for each locus and over all loci. Allele frequencies obtained in NS breed were compared
to the frequencies already available in literature for the Large White, Landrace, Duroc, Belgian Landrace, Piétrain,
Hampshire and Meishan breeds. For the ESR locus, as no information on the distribution of the two alleles were available,
we typed a sample of unrelated pigs from the considered breeds.
Even if only eight loci were studied in NS breed, important elements were obtained from the data. The 1843T (n) allele
at the RYR1 locus is present in NS breed, thus the molecular test to identify the carriers of this allele should be adopted
to avoid its spreading in the population. Moreover, other studies are needed to clarify the allelic structure of the MC1R
gene, which affects coat color, in order to evaluate if this gene could be used in genetic tests for the traceability of the
meat products of this breed. Finally, the present work represents an attempt to evaluate data on mutations within major
and candidate genes with the final aim to provide information that could be useful for the conservation and valorization
of local farm animal genetic resources.
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the Italian Journal of Animal Science [eISSN 1828-051X] is the official journal of the Animal Science and Production Association and it is published by PAGEPress®, Pavia, Italy. Reg. Pavia, n. 2/2010-INF. All credits and honors to PKP for their OJS.